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Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination… PAS 015 Anyone using cell-based products

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Description

Anyone using cell-based products

Who is BS 1635 - Recommendations for graphic symbols and abbreviations for

This part of ISO 4266 gives guidance on the accuracy

the requirements including performance requirements apply to the complete product

Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination… PAS 015 Anyone using cell-based products1 Scope This Technical Specification describes the identification of Shiga toxin producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae (References[2][3]); b) the genes associated with the serogroups O157, O111, O26, O103, and O145 (References[3][4]). In any case, when one or both of the stx genes is are detected, the isolation of the strain is attempted. The isolation of

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