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Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer
Dilute 20ml TAE Buffer 50X with 980ml deionised water to make 1 litre of TAE Buffer
10 pH (100 g/l) 2
Cold Spring Harbour laboratory
LBM-Broth Miller Size:1000g Dilute 50 ml MOPS-SDS 20XLBM Broth High Salt In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 26 gram powdered medium in 1 litre distilled water Store dry at room temperature