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is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element
32 KH2PO4 870 Carbon Source Glucose 10000 Total 20326 Suspend 20
5x is used in preparing M9 Minimal medium used for the cultivation and maintenance of E
Components
SOC Broth Size:1kg is omitted from this formulationComponent g l Glucose 3. 6 (20 mN) Tryptone 20 Yeast extract 5 NaCl 0. 5 MgSO4 2. 44 Final pH 7. 0 0. 2 at 25C Suspend 31. 5 gram powdered medium in 1 litre distilled water Store dry at room temperature.